石膏样小孢子菌感染小鼠的Dectin-1、TLR-2和TLR-4及细胞因子动态变化

doi:10.11843/j.issn.0366-6964.2019.07.014

Abstract

Abstract: The aim of this study was to investigate the dynamic changes of Dectin-1, TLR-2 and TLR-4 pattern recognition receptors and cytokines in skin lesions of mice infected with Microsporum gypseum. M. gypseum from giant panda were subcutaneously inoculated into the C57BL/6 mice. The skin tissues with pathological changes were collected on the 3rd, 7th and 14th day, then the spore colonization sites and pathological damages were observed by PAS staining and HE staining. The transcription of pattern recognition receptors mRNA were detected by real-time PCR at the 3rd, 6th and 12th hour. The transcription of cytokines mRNA were detected by real-time PCR at the 1st, 3rd, 7th and 14th day. The results showed that M. gypseum infected mice resulted in a small subcutaneous abscess, a large number of inflammatory cells infiltrated, and accompanied by thickening of the stratum corneum. The transcription of the pattern recognition receptors Dectin-1, TLR-2 and TLR-4 were increased (P<0.01), the transcription levels of cytokines IL-6, IL-1β, IL-23, TGF-β, IL-17A, IL-17F and IL-22 were increased (P<0.01). The pattern recognition receptor can recognize the surface pattern recognition molecules of M. gypseum and activate the Th17 pathway, and differentiate the mature Th17 cells into secret cell cytokines IL-22, IL-17A and IL-17F, thereby exerting resistance to M. gypseum.

CLC Number:

S852.661

YU Yan, GU Yu, WANG Chengdong, HU Jing, YANG Aining, YANG Qiao, LI Desheng, ZHANG Hemin, DENG Linhua, LING Shanshan, ZUO Zhicai, PENG Guangneng, ZHONG Zhijun, MA Xiaoping. Dynamic Changes of Dectin-1, TLR-2,TLR-4 and Cytokines in Mice Infected with Microsporum gypseum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1449-1457.

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https://www.xmsyxb.com/EN/Y2019/V50/I7/1449

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Dynamic Changes of Dectin-1, TLR-2,TLR-4 and Cytokines in Mice Infected with Microsporum gypseum

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